Escherichia coli LYC-1

Ancestors

Derived strains

• Escherichia coli LYC-2
• Escherichia coli LYC-3
• Escherichia coli LYC-4
• Escherichia coli LYC-5

Genotype with respect to wild type

F⁺ (λ) | F⁻ recA1, IN(rrnD-rrnE)1, rph-1, rpoB331(RifR), hsdR19, λ(DE3[lacI, lacUV5-T7 gene 1, ind1, sam7, nin5]), (Rif^r ) | Plasmid pRSFD-rtxA-D9 added

Bars (|) indicate differences between strains.

Plasmids

• pRSFD-rtxA-D9 (RSF1030 ori, lacI, P_T7lac-rtxA (residues 1-345 and 463 pb upstream of rtxA))

References

• Yuchang Luo, Qinqin Zhao, Qian Liu & Yan Feng (2019). An Artificial Biosynthetic Pathway for 2‑Amino-1,3-Propanediol Production Using Metabolically Engineered Escherichia coli. ACS Synthetic Biology.