Escherichia coli LYC-5

Ancestors

Genotype with respect to wild type

F⁺ (λ) | F⁻ recA1, IN(rrnD-rrnE)1, rph-1, rpoB331(RifR), hsdR19, λ(DE3[lacI, lacUV5-T7 gene 1, ind1, sam7, nin5]), (Rif^r ) | Plasmid pRSFD-rtxA-D9 added | Plasmid pACYCD-serBeco-gdhAeco added

Bars (|) indicate differences between strains.

Plasmids

pRSFD-rtxA-D9 (RSF1030 ori, lacI, P_T7lac-rtxA (residues 1-345 and 463 pb upstream of rtxA))

pACYCD-serBeco-gdhAeco (p15A ori, lacI P_T7lac-serB P_T7lac-gdhA)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
serinol	Titer	14.6 g/L	29.8 OD₆₀₀	Glucose	120 h	Fed-Batch	[278]
serinol	Volumetric productivity	0.122 g/L/h	29.8 OD₆₀₀	Glucose	120 h	Fed-Batch	[278]
serinol	Titer	3.4 g/L		Glucose		Flask	[278]
serinol	Substrate yield	0.316 mol/mol of substrate	29.8 OD₆₀₀	Glucose	120 h	Fed-Batch	[278]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Yuchang Luo, Qinqin Zhao, Qian Liu & Yan Feng (2019). An Artificial Biosynthetic Pathway for 2‑Amino-1,3-Propanediol Production Using Metabolically Engineered Escherichia coli. ACS Synthetic Biology.