Escherichia coli BuT-8LA

Ancestors

Derived strains

Escherichia coli BuT-8L-ato

Genotype with respect to parental

PλP_L-atoDABE

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | ΔptsGΔpoxB HK022::λP_RP_L-glf | ΔldhAΔfrdA ϕ80attB:: PλP_L-ter λattB:: PλP_L-crt ΔadhE::ϕ80attB:: PλP_L-pha-hbd | PλP_L-atoDABE

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
atoD	Escherichia coli	Overexpression
atoA	Escherichia coli	Overexpression
atoB	Escherichia coli	Overexpression
atoE	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
butanoate	Titer	3.4 g/L	7.5 OD₅₅₀^*	Glucose	24 h	Flask	[280]
butanoate	Titer	4.1 g/L	8.1 OD₅₅₀^*	Glucose acetate	24 h	Flask	[280]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Mukesh Saini, Zei Wen Wang, Chung-Jen Chiang & Yun-Peng Chao (2014). Metabolic Engineering of Escherichia coli for Production of Butyric Acid. Journal of Agricultural & Food Chemistry.