Escherichia coli BuT-8L-ato

Ancestors

Genotype with respect to parental

Δpta

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | ΔptsGΔpoxB HK022::λP_RP_L-glf | ΔldhAΔfrdA ϕ80attB:: PλP_L-ter λattB:: PλP_L-crt ΔadhE::ϕ80attB:: PλP_L-pha-hbd | PλP_L-atoDABE | Δpta

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
pta	Escherichia coli	Silencing

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
butanoate	Titer	2.5 g/L	7.7 OD₅₅₀^*	Glucose	24 h	Flask	[280]
butanoate	Titer	6.8 g/L	7.8 OD₅₅₀^*	Glucose acetate	24 h	Flask	[280]
butanoate	Titer	4.3 g/L ^*	8.2 OD₅₅₀^*	Glucose acetate	24 h	Flask	[280]
butanoate	Titer	5.6 g/L ^*	8.0 OD₅₅₀^*	Glucose acetate	24 h	Flask	[280]
butanoate	Titer	6.3 g/L ^*	7.7 OD₅₅₀^*	Glucose acetate	24 h	Flask	[280]
butanoate	Titer	10.6 g/L ^*	7.5 OD₅₅₀^*	Glucose acetate	48 h	Fed-Batch	[280]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Mukesh Saini, Zei Wen Wang, Chung-Jen Chiang & Yun-Peng Chao (2014). Metabolic Engineering of Escherichia coli for Production of Butyric Acid. Journal of Agricultural & Food Chemistry.