Escherichia coli BWT05

Ancestors

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pCDFDuet-AtCCR-Pc4CL-AtPAL added | Plasmid pET28a-UGT73C5 added

Bars (|) indicate differences between strains.

Plasmids

pCDFDuet-AtCCR-Pc4CL-AtPAL (CloDF13 ori, lacI, AtCCR, Pc4CL, AtPAL)

pET28a-UGT73C5 (pBR322 ori, f1 ori, P_T7-UGT73C5)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
cinnamyl alcohol	Titer	118.4 mg/L		Glucose	24 h	Flask	[283]
rosin	Titer	168.9 mg/L		Glucose	24 h	Flask	[283]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Zhou, Wei; Bi, Huiping; Zhuang, Yibin; He, Qinglin; Yin, Hua; Liu, Tao & Ma, Yanhe. Production of Cinnamyl Alcohol Glucoside from Glucose in Escherichia coli. Journal of Agricultural and Food Chemistry. 2017, 65(10), 2129-2135.