Escherichia coli ENB33

Ancestors

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | (DE3) | Plasmid pISP5 added | Plasmid pMAP6 added | Plasmid pNB33 added

Bars (|) indicate differences between strains.

Plasmids

• pMAP6 (pBR322 origin, P_T7-EfmvaE, P_T7-EfmvaS^A110G)

  pISP5 (P_T7-MmMK-ScPMK-ScPMD-ScIDI)

  pNB33 (P_T7-LdtBPPS^L526S-P_T7-SltNPPS)

References

• Dengwei Lei, Zetian Qiu, Jihua Wu, Bin Qiao, Jianjun Qiao & Guang-Rong Zhao (2021). Combining Metabolic and Monoterpene Synthase Engineering for de Novo Production of Monoterpene Alcohols in Escherichia coli. ACS Synthetic Biology.