Escherichia coli ET4

Ancestors

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | Plasmid pCF1s-MsD added | Plasmid pAC1c-hMsC/hMsE added | Plasmid pQE1a-mMsB/EcA added

Bars (|) indicate differences between strains.

Plasmids

pQE1a-mMsB/EcA (ColE1 ori, P_tac-MBP-egtB and gshA)

pAC1c-hMsC/hMsE (p15A ori, His-tagged egtC and egtE)

pCF1s-MsD (CDF ori, P_tac-egtD)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
ergothioneine	Titer	17.0 mg/L	8.7 OD₆₀₀	Glucose	72 h	Flask	[285]
hercynine	Titer	121.0 mg/L	8.7 OD₆₀₀	Glucose	72 h	Flask	[285]
γ-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide	Titer	1.0 mg/L	8.7 OD₆₀₀	Glucose	72 h	Flask	[285]
S-(hercyn-2-yl)-L-cysteine S-oxide	Titer	9.0 mg/L	8.7 OD₆₀₀	Glucose	72 h	Flask	[285]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Ryo Osawa, Tomoyuki Kamide, Yasuharu Satoh, Yusuke Kawano, Iwao Ohtsu & Tohru Dairi (2018). Heterologous and High Production of Ergothioneine in Escherichia coli. Journal of Agricultural & Food Chemistry.