Escherichia coli 050H (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A)
Ancestors
Genotype with respect to wild type
Δack-pta | ΔldhA | ΔadhE | frdA::T7RNAP | PmacAB::PT7 | Plasmid pMevΔFPPS added | Plasmid pET-FPPS-(PT)4P-PTS2C415F/H454A addedBars (|) indicate differences between strains.
Plasmids
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pMevΔFPPS (PlacUV5-atoB-HMGS-tHMGR-MK-PMK-PMDidi)
pET-FPPS-(PT)4P-PTS2C415F/H454A (ispA-(PT)4P linker-PTS2C415F/H454A)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| (-)-patchoulol | Titer | 321.4 mg/L * | Flask | [287] | |||
| (-)-patchoulol | Biomass-specific yield | 25.63 mg/g DCW * | Flask | [287] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.