Escherichia coli MGΔpgi
Ancestors
Genotype with respect to parental
Δpgi
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Δpgi | Plasmid pTrc99a-iaa9 added | Plasmid p6LRPW326 addedBars (|) indicate differences between strains.
Plasmids
-
p6LRPW326 (trp operon, aroGfbr, trpEfbr, and serAfbr)
pTrc99a-iaa9 (Ptrc-iaaM-Ptac-ami1-Ptac-ami1)
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Silencing
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tryptophan | Titer | 1.015 g/L | 48 h | Flask | [289] | ||
| (indol-3-yl)acetate | Titer | 0.8 g/L * | 96 h * | Flask | [289] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.
References
- Wu, Hongxuan; Yang, Jinhua; Shen, Peijie; Li, Qingchen; Wu, Weibin; Jiang, Xianzhang; Qin, Lina; Huang, Jianzhong; Cao, Xiao & Qi, Feng. High-Level Production of Indole-3-acetic Acid in the Metabolically Engineered Escherichia coli. Journal of Agricultural and Food Chemistry. 2021, 69(6), 1916-1924.