Escherichia coli MGΔpgiΔgdhA

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli MGΔpgiΔgdhA

Genotype with respect to parental

Δpgi ΔgdhA

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Δpgi ΔgdhA | Plasmid pTrc99a-iaa9 added | Plasmid p6LRPW326 added

Bars (|) indicate differences between strains.

Plasmids

p6LRPW326 (trp operon, aroG^fbr, trpE^fbr, and serA^fbr)

pTrc99a-iaa9 (P_trc-iaaM-P_tac-ami1-P_tac-ami1)

Modifications

Gene	Organism	Modification
pgi	Escherichia coli	Silencing
gdhA	Escherichia coli	Silencing

References

Wu, Hongxuan; Yang, Jinhua; Shen, Peijie; Li, Qingchen; Wu, Weibin; Jiang, Xianzhang; Qin, Lina; Huang, Jianzhong; Cao, Xiao & Qi, Feng. High-Level Production of Indole-3-acetic Acid in the Metabolically Engineered Escherichia coli. Journal of Agricultural and Food Chemistry. 2021, 69(6), 1916-1924.