Escherichia coli MGΔldhA::mdh

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli MGΔldhA::mdh

Genotype with respect to parental

ΔldhA::mdh

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔldhA::mdh | Plasmid pTrc99a-iaa9 added | Plasmid p6LRPW326 added

Bars (|) indicate differences between strains.

Plasmids

p6LRPW326 (trp operon, aroG^fbr, trpE^fbr, and serA^fbr)

pTrc99a-iaa9 (P_trc-iaaM-P_tac-ami1-P_tac-ami1)

Modifications

Gene	Organism	Modification
ldhA	Escherichia coli	Silencing
mdh	Escherichia coli	Overexpression

References

Wu, Hongxuan; Yang, Jinhua; Shen, Peijie; Li, Qingchen; Wu, Weibin; Jiang, Xianzhang; Qin, Lina; Huang, Jianzhong; Cao, Xiao & Qi, Feng. High-Level Production of Indole-3-acetic Acid in the Metabolically Engineered Escherichia coli. Journal of Agricultural and Food Chemistry. 2021, 69(6), 1916-1924.