Escherichia coli W-126-GA(M)

Ancestors

Derived strains

Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | alaDGs | Δpta ΔldhA ΔadhE ΔpflB | Plasmid PJ23105-alaE added

Bars (|) indicate differences between strains.


Plasmids

Strain derived from a randomly mutated strain.


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-alanine Titer 24.8 g/L Flask [293]
L-alanine Biomass-specific yield 3.41 mg/g DCW Flask [293]
L-alanine Titer 80.2 g/L Batch [293]
L-alanine Substrate yield 0.57 g/g of sustrate Batch [293]
L-alanine Volumetric productivity 2.23 g/L/h Batch [293]
acetate Titer 8.62 g/L Batch [293]
L-alanine Titer 22.3 g/L 3.91 OD600 Batch [293]
L-alanine Titer 102.5 g/L Batch [293]
L-alanine Substrate yield 0.84 g/g of sustrate Batch [293]
L-alanine Volumetric productivity 4.27 g/L/h Batch [293]
acetate Titer 1.42 g/L Batch [293]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References