Escherichia coli W-PAla-7-BetT
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | alaDGs | Δpta ΔldhA ΔadhE ΔpflB | Plasmid PJ23105-alaE added | Plasmid pTET-PAla-7-BetT addedBars (|) indicate differences between strains.
Plasmids
-
PJ23105-alaE (p15A ori, Ptac, gapA, PJ23105, alaE)
pTET-PAla-7-BetT (p15A ori, PAla-7, BetT)
Strain derived from a randomly mutated strain.
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-alanine | Titer | 134.5 g/L | 24 h | Batch | [293] | ||
| L-alanine | Volumetric productivity | 5.6 g/L/h | 24 h | Batch | [293] | ||
| L-alanine | Substrate yield | 0.89 g/g of sustrate | Flask | [293] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.