Escherichia coli ALS929 pTrc99A-alaD

Ancestors

Genotype with respect to wild type
[Hfr zbi::Tn10 poxB1 D (aceEF) rpsL pps-4 pfl-1] | ldhA::- Kan | Plasmid pTrc99A-alaD added

Bars (|) indicate differences between strains.


Plasmids

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
acetate Titer 10.0 g/L 5.3 g/L 9 h Batch [295]
L-alanine Titer 34.0 g/L 5.3 g/L 13 h Batch [295]
L-alanine Substrate yield 0.86 g/g of sustrate 5.3 g/L 13 h Batch [295]
acetate Titer 1.0 g/L 5.6 g/L 25 h Fed-Batch [295]
L-alanine Titer 88.0 g/L 5.6 g/L 48 h Fed-Batch [295]
L-alanine Volumetric productivity 4.0 g/L/h 5.6 g/L 48 h Fed-Batch [295]
L-alanine Substrate yield 1.0 g/g of sustrate 5.6 g/L 48 h Fed-Batch [295]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References