Escherichia coli N9

Ancestors

All strains

Escherichia coli K12

Escherichia coli BW25113

Escherichia coli N1

Escherichia coli N2

Escherichia coli N3

Escherichia coli N4

Escherichia coli N9

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | ΔargA | ΔspeF ΔspeB | ΔastA | ΔargR | Plasmid p-ARG-2^a added

Bars (|) indicate differences between strains.

Plasmids

p-ARG-2^a (P_LlacO1-carAB-argI-argG-argH)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-arginine	Titer	7.6 g/L	13.3 OD₆₀₀	Glucose ornithine		Flask	[300]
L-arginine	Substrate yield	0.76 mol/mol of substrate	13.3 OD₆₀₀	ornithine		Flask	[300]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Nie, Mengzhen; Wang, Jingyu & Zhang, Kechun. A novel strategy for l-arginine production in engineered Escherichia coli. Microbial Cell Factories. 2023, 22(1).