Escherichia coli Arg4

Ancestors

Derived strains

Genotype with respect to parental

artJ::P_BJ23119-B0034-lysE^Cg and argT::P_BJ23119-B0034-cgmA^Cg

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | argA^K204T, argR^Y33X, pyrG^D450H, and speB^E189A | △speC △speF | argE^Ec::P_BJ23119-B0034-argJ^Tn | yghX::P_BJ23119-B0034-argCBH^Ec, yncI::P_BJ23119-B0034-argA*, rpnD::P_BJ23119-B0034-argD, ycjV::P_BJ23119-B0034-argI and yrfG::P_BJ23119-B0034-argG | artJ::P_BJ23119-B0034-lysE^Cg and argT::P_BJ23119-B0034-cgmA^Cg

Bars (|) indicate differences between strains.

Strain derived from a randomly mutated strain.

Modifications

Gene	Organism	Modification
artJ	Escherichia coli	Silencing
lysE	Corynebacterium glutamicum	Overexpression
argT	Escherichia coli	Silencing
lysG	Corynebacterium glutamicum	Overexpression

Production

Metabolites	Production type	Production	Carbon source	Time	Scale	Ref.
L-arginine	Titer	52.83 g/L	Glucose	48 h ^*	Fed-Batch	[301]
L-arginine	Substrate yield	0.3 g/g of sustrate	Glucose	48 h ^*	Fed-Batch	[301]
L-arginine	Volumetric productivity	1.1 g/L/h	Glucose	48 h ^*	Fed-Batch	[301]
L-citrulline	Titer	9.91 g/L	Glucose	48 h	Fed-Batch	[301]
L-arginine	Titer	78.11 g/L	Glucose		Fed-Batch	[301]
L-arginine	Titer	64.02 g/L	Glucose		Fed-Batch	[301]
L-arginine	Titer	58.46 g/L	Glucose		Fed-Batch	[301]
L-citrulline	Titer	3.31 g/L	Glucose		Fed-Batch	[301]
L-citrulline	Titer	4.98 g/L	Glucose		Fed-Batch	[301]
L-citrulline	Titer	6.85 g/L	Glucose		Fed-Batch	[301]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Sheng, Qi; He, Shengyang; Liang, Guangjie; Meng, Gang; Zhao, Chunguang; Wei, Aiying; Gou, Lining; Liu, Jia; Li, Xiaomin; Wu, Jing & Liu, Liming. Improved arginine production in Escherichia coli by harnessing the intracellular citrulline. Metabolic Engineering. 2025.