Escherichia coli Arg1-3

Ancestors

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | argA^K204T, argR^Y33X, pyrG^D450H, and speB^E189A | △speC △speF | Plasmid pCDR-P_BJ23119-B0034-argJ^Bs added

Bars (|) indicate differences between strains.

Plasmids

• pCDR-P_BJ23119-B0034-argJ^Bs (CloDF13 ori, P_BJ23119-B0034-argJ^Bs)

Strain derived from a randomly mutated strain.

References

• Sheng, Qi; He, Shengyang; Liang, Guangjie; Meng, Gang; Zhao, Chunguang; Wei, Aiying; Gou, Lining; Liu, Jia; Li, Xiaomin; Wu, Jing & Liu, Liming. Improved arginine production in Escherichia coli by harnessing the intracellular citrulline. Metabolic Engineering. 2025.