Escherichia coli T16

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli TRTH

Escherichia coli T03

Escherichia coli T12

Escherichia coli T15

Escherichia coli T16

Genotype with respect to parental

P_ara-aceB-mdh-pck::yghxΔppc

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | trpEDCBA+Tet^R, ΔtnaA | P_acs-acs::ynci | P_acs-acs::ycjv | P_ara-aceB-mdh-pck::yghxΔppc

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
aceB	Escherichia coli	Overexpression
mdh	Escherichia coli	Overexpression
pck	Escherichia coli	Overexpression
ppc	Escherichia coli	Silencing

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan	Titer	54.6 g/L	35.5 g/L	Glucose		Fed-Batch	[170]
acetate	Titer	1.4 g/L	35.3 g/L	Glucose		Fed-Batch	[170]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Du, L., Zhang, Z., Xu, Q., & Chen, N. (2019). New strategy for removing acetic acid as a by-product during L-tryptophan production. Biotechnology & Biotechnological Equipment, 33(1), 1471–1480.