Escherichia coli Arg4-5

Ancestors

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | argA^K204T, argR^Y33X, pyrG^D450H, and speB^E189A | △speC △speF | argE^Ec::P_BJ23119-B0034-argJ^Tn | yghX::P_BJ23119-B0034-argCBH^Ec, yncI::P_BJ23119-B0034-argA*, rpnD::P_BJ23119-B0034-argD, ycjV::P_BJ23119-B0034-argI and yrfG::P_BJ23119-B0034-argG | artJ::P_BJ23119-B0034-lysE^Cg and argT::P_BJ23119-B0034-cgmA^Cg | Plasmid pCDR-P_BJ23119-B0034-aspC added

Bars (|) indicate differences between strains.

Plasmids

pCDR-P_BJ23119-B0034-aspC (CloDF13 ori, P_BJ23119-B0034-aspC)

Strain derived from a randomly mutated strain.

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-arginine	Titer	16.09 g/L		Glucose		Flask	[301]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Sheng, Qi; He, Shengyang; Liang, Guangjie; Meng, Gang; Zhao, Chunguang; Wei, Aiying; Gou, Lining; Liu, Jia; Li, Xiaomin; Wu, Jing & Liu, Liming. Improved arginine production in Escherichia coli by harnessing the intracellular citrulline. Metabolic Engineering. 2025.