Escherichia coli Arg4-7
Ancestors
Genotype with respect to parental
CRISPRi-downregulated pyrF
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | argAK204T, argRY33X, pyrGD450H, and speBE189A | △speC △speF | argEEc::PBJ23119-B0034-argJTn | yghX::PBJ23119-B0034-argCBHEc, yncI::PBJ23119-B0034-argA*, rpnD::PBJ23119-B0034-argD, ycjV::PBJ23119-B0034-argI and yrfG::PBJ23119-B0034-argG | artJ::PBJ23119-B0034-lysECg and argT::PBJ23119-B0034-cgmACg | CRISPRi-downregulated pyrFBars (|) indicate differences between strains.
Strain derived from a randomly mutated strain.
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Attenuation
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-arginine | Titer | 15.76 g/L | Flask | [301] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.