Escherichia coli Arg9-4

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli Arg0

Escherichia coli Arg1

Escherichia coli Arg2

Escherichia coli Arg3

Escherichia coli Arg4

Escherichia coli Arg5

Escherichia coli Arg6

Escherichia coli Arg7

Escherichia coli Arg8

Escherichia coli Arg9

Escherichia coli Arg9-4

Genotype with respect to parental

P_BJ23115-B0034-pyrF and P_BJ23110-B0034-argF

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | argA^K204T, argR^Y33X, pyrG^D450H, and speB^E189A | △speC △speF | argE^Ec::P_BJ23119-B0034-argJ^Tn | yghX::P_BJ23119-B0034-argCBH^Ec, yncI::P_BJ23119-B0034-argA*, rpnD::P_BJ23119-B0034-argD, ycjV::P_BJ23119-B0034-argI and yrfG::P_BJ23119-B0034-argG | artJ::P_BJ23119-B0034-lysE^Cg and argT::P_BJ23119-B0034-cgmA^Cg | yacC::P_BJ23119-B0034-gdhA^St and ydjK::P_BJ23119-B0034-gdhA^St | P_icd:P_BJ23100-B0034 | P_ppc:P_BJ23114-B0034 | P_BJ23110-B0034-aspC and P_BJ23115-B0034-gltA | yafF::P_BJ23119-B0034-glnA^Bs | P_BJ23115-B0034-pyrF and P_BJ23110-B0034-argF

Bars (|) indicate differences between strains.

Strain derived from a randomly mutated strain.

Modifications

Gene	Organism	Modification
pyrF	Escherichia coli	Attenuation
argF	Escherichia coli	Overexpression

References

Sheng, Qi; He, Shengyang; Liang, Guangjie; Meng, Gang; Zhao, Chunguang; Wei, Aiying; Gou, Lining; Liu, Jia; Li, Xiaomin; Wu, Jing & Liu, Liming. Improved arginine production in Escherichia coli by harnessing the intracellular citrulline. Metabolic Engineering. 2025.