Escherichia coli ARG12

Ancestors

Derived strains

Genotype with respect to parental

ilvG::P_trc-lysE

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔspeA ΔadiA ΔastA | ΔlacI ΔargR yncI::P_trc-argJ | yghX::P_trc-argC-argJ-argB-argD-argF, ygaY::P_trc-argG-argH | argE::P_trc-argJ | ycjV::P_trc-pyrAA/pyrABE949* | yjiP::pntAB | P_sucA::P_{rpsT P1} | ilvG::P_trc-lysE

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
ilvG	Escherichia coli	Silencing
lysE	Corynebacterium glutamicum	Overexpression

Production

Metabolites	Production type	Production	Carbon source	Scale	Ref.
L-arginine	Titer	28.3 g/L	Glucose	Flask	[307]
L-arginine	Titer	110.0 g/L	Glucose	Fed-Batch	[307]
L-arginine	Substrate yield	0.43 g/g of sustrate	Glucose	Fed-Batch	[307]
L-arginine	Volumetric productivity	2.29 g/L/h	Glucose	Fed-Batch	[307]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Shuai Jiang, Ruirui Wang, Dehu Wang, Chunguang Zhao, Qian Ma, Heyun Wu & Xixian Xie (2023). Metabolic reprogramming and biosensor-assisted mutagenesis screening for high-level production of L-arginine in Escherichia coli. Metabolic Engineering.