Escherichia coli W3110 pCys

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid pCys added

Bars (|) indicate differences between strains.


Plasmids

  • pCys (PserA1,2-serAfbr, PcysE-cysEfbr, PGAPDH-ydeD)


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-cysteine Titer 16.2 g/L 48 h Fed-Batch [313]
N-acetyl-L-serine Titer 29.9 g/L 48 h Fed-Batch [313]
L-cysteine Biomass-specific productivity 16.5 mg/g DCW/h 21 h Fed-Batch [313]
L-cysteine Substrate yield 4.96 % Fed-Batch [313]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References