Escherichia coli ΔcysM/pDES/pCA24N
Ancestors
Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | cysM::KmR | Plasmid pCA24N added | Plasmid pDES addedBars (|) indicate differences between strains.
Plasmids
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-cysteine | Titer | 55.81 mg/L * | 72 h * | Flask | [319] | ||
| L-cysteine | Titer | 63.07 mg/L * | 72 h * | Flask | [319] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.
References
- Takeshi Nakatani, Iwao Ohtsu, Gen Nonaka, Natthawut Wiriyathanawudhiwong, Susumu Morigasaki & Hiroshi Takagi (2012). Enhancement of thioredoxin/glutaredoxin-mediated L-cysteine synthesis from S-sulfocysteine increases L-cysteine production in Escherichia coli. Microbial Cell Factories.
- Kawano, Yusuke; Onishi, Fumito; Shiroyama, Maeka; Miura, Masashi; Tanaka, Naoyuki; Oshiro, Satoshi; Nonaka, Gen; Nakanishi, Tsuyoshi & Ohtsu, Iwao. Improved fermentative l-cysteine overproduction by enhancing a newly identified thiosulfate assimilation pathway in Escherichia coli. Applied Microbiology and Biotechnology. 2017, 101(18), 6879-6889.