Escherichia coli ΔpykF/ptsH

Ancestors

Genotype with respect to parental

ΔptsH::FRT


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR | ΔtnaA | ΔpheA | ΔtyrA::FRT | Δpta::pta1 | Plasmid pSV03 added | ΔpykF::FRT | ΔptsH::FRT

Bars (|) indicate differences between strains.


Plasmids

  • pSV03 (p15A ori, aroFfbr trpEfbrD)


Modifications
Gene Organism Modification
Silencing

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-tryptophan Titer 0.78 g/L 4.4 OD600 48 h Flask [175]
L-tryptophan Substrate yield 0.128 g/g of sustrate 4.4 OD600 48 h Flask [175]
acetate Titer 0.97 g/L 4.4 OD600 48 h Flask [175]
L-tryptophan Titer 3.8 g/L 21.6 OD600 Fed-Batch [175]
L-tryptophan Substrate yield 0.073 g/g of sustrate 21.6 OD600 Fed-Batch [175]
acetate Titer 0.2 g/L 21.6 OD600 Fed-Batch [175]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References