Escherichia coli Xllp17

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli HD-1

Escherichia coli Xllp02

Escherichia coli Xllp04

Escherichia coli Xllp08

Escherichia coli Xllp17

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | P_PL-pheA^Thr326P-aroF | ΔptsH | P_m37-galP P_m93-glk | tyrR^T495I | Plasmid p-ydiB added

Bars (|) indicate differences between strains.

Plasmids

p-ydiB (P_trc-ydiB)

Strain derived from a randomly mutated strain.

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-phenylalanine	Titer	3.67 g/L	8.4 OD₆₀₀	Glucose		Flask	[320]
L-phenylalanine	Substrate yield	0.08 g/g of sustrate	8.4 OD₆₀₀	Glucose		Flask	[320]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Yongfei Liu, Yiran Xu, Dongqin Ding, Jianping Wen, Beiwei Zhu & Dawei Zhang (2018). Genetic engineering of Escherichia coli to improve L-phenylalanine production. BMC Biotechnology.