Escherichia coli VAL32

Ancestors

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | P_ilvC::P_trc | P_ilvDE::P_trc | △brnQ | brnQ::brnFE | yeeL::P_trc-PdhR | araBAD::dCas9 | Plasmid pGRB-RpoS-3 added

Bars (|) indicate differences between strains.

Plasmids

• pGRB-RpoS-3 (gRNA targeting the rpoS gene)

Strain derived from a randomly mutated strain.

References

• Yanan Hao, Xuewei Pan, Rufan Xing, Jiajia You, Mengkai Hu, Zhifei Liu, Xiangfei Li, Meijuan Xu & Zhiming Rao (2022). High-level production of L-valine in Escherichia coli using multi-modular engineering. Bioresource Technology.