Escherichia coli VAL33
Ancestors
Derived strains
Genotype with respect to parental
trpR::Ptrc-edd-eda
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | PilvC::Ptrc | PilvDE::Ptrc | △brnQ | brnQ::brnFE | yeeL::Ptrc-PdhR | araBAD::dCas9 | Plasmid pGRB-RpoS-1 added | trpR::Ptrc-edd-edaBars (|) indicate differences between strains.
Plasmids
-
pGRB-RpoS-1 (gRNA targeting the rpoS gene)
Strain derived from a randomly mutated strain.
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Silencing
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-valine | Titer | 21.25 g/L | 24 h | Flask | [321] | ||
| L-valine | Substrate yield | 0.33 g/g of sustrate | 24 h | Flask | [321] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.