Escherichia coli PHE12
Ancestors
Derived strains
Genotype with respect to parental
ΔylbE::pycP458S::PrrnC-pykFΔdhaL::pckA
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | L-tyrosine auxotrophic | Plasmid p15A::pL-aroF-tyrA1-121-pR-pheAT326P added | ΔfurR::furRE173K | Plasmid p15A::pL-aroF-tyrA1-121-pR-pheAT326P lost | ΔdadX-cvrA:: PJ23109-aroFfbr-PJ23107-pheAfbr::PJ23107-aroE-PJ23110-aroD | ΔzwfΔtktAΔtktB::Ptac-xfspK | ΔylbE::pycP458S::PrrnC-pykFΔdhaL::pckABars (|) indicate differences between strains.
Strain derived from a randomly mutated strain.
Modifications
| Gene | Organism | Modification |
|---|---|---|
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Silencing
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Feedback insensitive
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Attenuation
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Silencing
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-phenylalanine | Titer | 92.21 g/L | 100.5 OD610 | 58 h | Fed-Batch | [322] | |
| L-phenylalanine | Substrate yield | 0.227 g/g of sustrate | 100.5 OD610 | 58 h | Fed-Batch | [322] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.