Escherichia coli DA-29
Ancestors
Genotype with respect to parental
yncI::Ptrc-gapA
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | lacI::PxylA-T7 RNA Polymerase, △tynA | ylbE::Ptrc-hpaBC, yjiK::PT7-DmDdc | △tyrR, ygaY::Ptrc-aroGfbr, ycgH::Ptrc-tyrAfbr | yciQ::Ptrc-aroE, yeeL::Ptrc-tyrB | △pykF | yncK::Ptrc-pps | yeeP::Ptrc-tktA, yjgX::Ptrc-talB | yghE::Ptrc-hpaBC, mbhA::PT7-DmDdc | gapC::Ptrc-hpaBC, rph::PT7-DmDdc | ilvG::Ptrc-hpaBC, ycdN::PT7-DmDdc | yjiV::Ptrc-fre | △adhE | yncI::Ptrc-gapABars (|) indicate differences between strains.
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Silencing
|
||
|
Overexpression
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| dopamine | Titer | 22.58 g/L | Fed-Batch | [323] | |||
| dopamine | Substrate yield | 0.0337 mol/mol of substrate | Fed-Batch | [323] | |||
| dopamine | Titer | 5.71 g/L | Flask | [323] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.