Escherichia coli EF-4

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli EF-1

Escherichia coli EF-2-2

Escherichia coli EF-2

Escherichia coli EF-3

Escherichia coli EF-4

Derived strains

Escherichia coli EF-5-1

Genotype with respect to parental

ribF::P_J23111-ribC^G199D

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | △lacI | Plasmid pduet-rib added | △pfkA | △edd△eda | △ribB-FMN riboswitch | ribF::P_J23111-ribC^G199D

Bars (|) indicate differences between strains.

Plasmids

pduet-rib (P_tac-ribEDAH)

Modifications

Gene	Organism	Modification
ribF	Escherichia coli	Silencing
ribC	Bacillus subtilis	Attenuation

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
riboflavin	Titer	660.0 mg/L		Glucose		Flask	[324]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Zhang, Xiaoling; Li, Yanan; Wang, Kang; Yin, Jilong; Du, Yuxuan; Yang, Zhen; Pan, Xuewei; You, Jiajia & Rao, Zhiming. Construction of antibiotic-free riboflavin producer in Escherichia coli by metabolic engineering strategies with a plasmid stabilization system. Synthetic and Systems Biotechnology. 2025, 10(2), 346-355.