Escherichia coli EF-5-1

Ancestors

Derived strains

• Escherichia coli EF-5

Genotype with respect to parental

△purR

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | △lacI | Plasmid pduet-rib added | △pfkA | △edd△eda | △ribB-FMN riboswitch | ribF::P_J23111-ribC^G199D | △purR

Bars (|) indicate differences between strains.

Plasmids

• pduet-rib (P_tac-ribEDAH)

References

• Zhang, Xiaoling; Li, Yanan; Wang, Kang; Yin, Jilong; Du, Yuxuan; Yang, Zhen; Pan, Xuewei; You, Jiajia & Rao, Zhiming. Construction of antibiotic-free riboflavin producer in Escherichia coli by metabolic engineering strategies with a plasmid stabilization system. Synthetic and Systems Biotechnology. 2025, 10(2), 346-355.