Escherichia coli EF-13

Ancestors

Derived strains

• Escherichia coli EF-15

Genotype with respect to parental

yghX::P_tac-pgl

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | △lacI | Plasmid pduet-rib added | △pfkA | △edd△eda | △ribB-FMN riboswitch | ribF::P_J23111-ribC^G199D | △purR | ybeM::P_tac-prs-purF | yncI::P_tac-gmk-ndk | ycgH::P_tac-rpiA-rpiB | yghX::P_tac-pgl

Bars (|) indicate differences between strains.

Plasmids

• pduet-rib (P_tac-ribEDAH)

References

• Zhang, Xiaoling; Li, Yanan; Wang, Kang; Yin, Jilong; Du, Yuxuan; Yang, Zhen; Pan, Xuewei; You, Jiajia & Rao, Zhiming. Construction of antibiotic-free riboflavin producer in Escherichia coli by metabolic engineering strategies with a plasmid stabilization system. Synthetic and Systems Biotechnology. 2025, 10(2), 346-355.