Escherichia coli dVio11

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli Mut

Escherichia coli Trp

Escherichia coli dVio9

Escherichia coli dVio11

Derived strains

Genotype with respect to parental

ptsHIcrr::P_{BBa_J23118}-glf

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid pDVIO-9 added | ptsHIcrr::P_{BBa_J23118}-glf

Bars (|) indicate differences between strains.

Plasmids

pDVIO-9 (P_trc-Optimized RBS 2-vioABCE^opt)

Strain derived from a randomly mutated strain.

Modifications

Gene	Organism	Modification
ptsH	Escherichia coli	Silencing
ptsI	Escherichia coli	Silencing
crr	Escherichia coli	Silencing
glf	Zymomonas mobilis	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
deoxyviolacein	Titer	0.87 g/L		Glucose		Flask	[325]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Tang, Mi; Pan, Zhenhui; Jin, Minghui; Zhang, Hengwei; Pan, Xuewei & Rao, Zhiming. Systems metabolic engineering of Escherichia coli for the high-level production of deoxyviolacein, a natural colorant. Bioresource Technology. 2025, 431, 132584.