Escherichia coli dVio20

Ancestors

Derived strains

Genotype with respect to parental

ilvG::P_trc-trpE^Q71K

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid pDVIO-9 added | ptsHIcrr::P_{BBa_J23118}-glf | ycdN::P_{BBa_J23105}-glk | ΔpykA | yjiP::P_trc-aroG^Q151F | yjiT::P_trc-aroCBD | ilvG::P_trc-trpE^Q71K

Bars (|) indicate differences between strains.

Plasmids

pDVIO-9 (P_trc-Optimized RBS 2-vioABCE^opt)

Strain derived from a randomly mutated strain.

Modifications

Gene	Organism	Modification
ilvG	Escherichia coli	Silencing
trpE	Escherichia coli	Feedback insensitive

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
deoxyviolacein	Titer	2.85 g/L		Glucose		Flask	[325]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Tang, Mi; Pan, Zhenhui; Jin, Minghui; Zhang, Hengwei; Pan, Xuewei & Rao, Zhiming. Systems metabolic engineering of Escherichia coli for the high-level production of deoxyviolacein, a natural colorant. Bioresource Technology. 2025, 431, 132584.