Escherichia coli dVio25

Ancestors

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid pDVIO-9 added | ptsHIcrr::P_{BBa_J23118}-glf | ycdN::P_{BBa_J23105}-glk | ΔpykA | yjiP::P_trc-aroG^Q151F | yjiT::P_trc-aroCBD | ilvG::P_trc-trpE^Q71K | Plasmid pDVIO-9 lost | Plasmid pDeut-T5 added

Bars (|) indicate differences between strains.

Plasmids

pDeut-T5 (P_{BBa_J23105}-aroG^Q151F-ppsA-tktA with TIGRs (Sample 5))

Strain derived from a randomly mutated strain.

Production

Metabolites	Production type	Production	Carbon source	Time	Scale	Ref.
deoxyviolacein	Titer	12.18 g/L	Glucose	72 h	Fed-Batch	[325]
deoxyviolacein	Titer	3.76 g/L	Glucose		Flask	[325]
deoxyviolacein	Volumetric productivity	0.169 g/L/h	Glucose	72 h	Fed-Batch	[325]
deoxyviolacein	Titer	8.2 g/L	Glycerol	72 h	Fed-Batch	[325]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Tang, Mi; Pan, Zhenhui; Jin, Minghui; Zhang, Hengwei; Pan, Xuewei & Rao, Zhiming. Systems metabolic engineering of Escherichia coli for the high-level production of deoxyviolacein, a natural colorant. Bioresource Technology. 2025, 431, 132584.