Escherichia coli DuetΔptsG

Ancestors

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | L-threonine producer | Plasmid pACYCDuet-1 added

Bars (|) indicate differences between strains.

Plasmids

• pACYCDuet-1 (p15A ori, lacI P_T7lac P_T7lac)

Strain derived from a randomly mutated strain.

References

• Ruxin Hao, Sumeng Wang, Xin Jin, Xiaoya Yang, Qingsheng Qi & Quanfeng Liang (2023). Dynamic and balanced regulation of the thrABC operon gene for efficient synthesis of L-threonine. Frontiers in Bioengineering and Biotechnology.