Escherichia coli W3110-TAA07
Ancestors
Genotype with respect to parental
Δicd
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | (DE3) | ∆frdA | ΔldhA | ΔpoxB | Δpta-ack | ΔpflB | ΔadhE | Plasmid pETDuet-adi1-tbrB added | Δycgh::PT7pyc | ΔadhE::PT7gltA | ΔicdBars (|) indicate differences between strains.
Plasmids
-
pETDuet-adi1-tbrB (adi1 and tbrB)
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Silencing
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| trans-aconitate | Substrate yield | 0.69 g/g of sustrate | Flask | [328] | |||
| trans-aconitate | Titer | 5.48 g/L | Flask | [328] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.
References
- Leilei Guo, Yi Cheng, Xiaoxu Tan, Hongxu Zhang, Hairong Yu, Wenjia Tian, Mingyuan Liu, Weikang Sun, Kaiyu Gao, Tianyi Jiang, Qianjin Kang, Wensi Meng, Yidong Liu, Zhaoqi Kang, Chuanjuan Lü, Chao Gao, Ping Xu & Cuiqing Ma (2025). Efficient trans-aconitic acid production using systematically metabolic engineered Escherichia coli. Synthetic and Systems Biotechnology.