Escherichia coli ZY17
Ancestors
Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | Δzwf::Cg-zwfA243T | ΔgndA::Cg-gndS361F | rpiA and rpiB | ΔpncC | ΔpfkA | Plasmid C1 added | Plasmid pRSFDuet-BmPnuC-BcNiaP addedBars (|) indicate differences between strains.
Plasmids
-
C1 (VpnadV -ΔPT7::PJ23119 ΔRBS::RBSIII-EcprsD115S)
pRSFDuet-BmPnuC-BcNiaP (BmPnuC and BcNiaP)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| β-nicotinamide D-ribonucleotide | Titer | 1.61 g/L | 10.0 OD600 | 10 h | Flask | [329] | |
| β-nicotinamide D-ribonucleotide | Titer | 13.3 g/L | 40.0 OD600 | 10 h | Fed-Batch | [329] | |
| β-nicotinamide D-ribonucleotide | Volumetric productivity | 1.11 g/L/h | 40.0 OD600 | 10 h | Fed-Batch | [329] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.