Escherichia coli CWF4NA/pTac15kPTA/pSynPPC7

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔiclR ΔfumC ΔfumA ΔfumB ΔptsG ΔlacI | PaspA::Ptrc | Plasmid pSynPPC7 added | Plasmid pTac15kPTA added

Bars (|) indicate differences between strains.


Plasmids

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
β-alanine Titer 3.94 g/L Flask [330]
β-alanine Substrate yield 0.25 g/g of sustrate Flask [330]
fumarate Titer 1.44 g/L Flask [330]
β-alanine Titer 20.1 g/L 65.7 OD600 33.5 h Fed-Batch [330]
β-alanine Volumetric productivity 0.457 g/L/h 65.7 OD600 33.5 h Fed-Batch [330]
fumarate Titer 0.0 g/L 65.7 OD600 33.5 h Fed-Batch [330]
acetate Titer 15.6 g/L 65.7 OD600 33.5 h Fed-Batch [330]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References