Escherichia coli CWF4NA2/pTac15kPTA/pSynPPC7
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔiclR ΔfumC ΔfumA ΔfumB ΔptsG ΔlacI | PaspA::Ptrc | Pacs::Ptrc | Plasmid pSynPPC7 added | Plasmid pTac15kPTA addedBars (|) indicate differences between strains.
Plasmids
-
pTac15kPTA (Ptac-RBS(ptrc)-panD-aspA)
pSynPPC7 (PJ23100-RBSptrc-ppc)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| β-alanine | Titer | 32.3 g/L | 97.1 OD600 | 33.5 h | Fed-Batch | [330] | |
| β-alanine | Substrate yield | 0.135 g/g of sustrate | 97.1 OD600 | 33.5 h | Fed-Batch | [330] | |
| β-alanine | Volumetric productivity | 0.828 g/L/h | 97.1 OD600 | 33.5 h | Fed-Batch | [330] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.