Escherichia coli S12

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli S03

Escherichia coli S12

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Δpta-ackA ΔldhA ΔadhE | Plasmid pADCTK added

Bars (|) indicate differences between strains.

Plasmids

pADCTK (p15A ori, P_trc, patAD-cadA-gabT-yahK)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
5-hydroxypentanoate	Titer	0.37 g/L		L-lysine hydrochloride		Flask	[331]
5-aminopentanoate	Titer	1.72 g/L		L-lysine hydrochloride		Flask	[331]
glutarate	Titer	0.49 g/L		L-lysine hydrochloride		Flask	[331]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Cen, Xuecong; Liu, Yanjuan; Zhu, Fanghuan; Liu, Dehua & Chen, Zhen. Metabolic engineering of Escherichia coli for high production of 1,5-pentanediol via a cadaverine-derived pathway. Metabolic Engineering. 2022, 74, 168-177.