Escherichia coli AC04

Ancestors

Derived strains

Genotype with respect to parental

△gdhA


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | High lysine-producing strain | Plasmid pLADH added | Plasmid pLADH lost | Plasmid pADCTKC added | Plasmid pBD added | △gabD | △gdhA

Bars (|) indicate differences between strains.


Plasmids

  • pBD (car_Mm-sfp-yqhD)

    pADCTKC (p15A ori, Ptrc, patAD-cadA-gabT-yahK-cgl0841)


Strain derived from a randomly mutated strain.


Modifications
Gene Organism Modification
Silencing

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
1,5-pentanediol Titer 2.62 g/L Flask [331]
cadaverine Titer 1.56 g/L Flask [331]
1,5-pentanediol Titer 9.25 g/L 172 g Fed-Batch [331]
1,5-pentanediol Substrate yield 0.16 g/g of sustrate 172 h Fed-Batch [331]
1,5-pentanediol Substrate yield 0.28 mol/mol of substrate 172 h Fed-Batch [331]
cadaverine Titer 2.23 g/L 74 h Fed-Batch [331]
cadaverine Titer 0.44 g/L 172 h Fed-Batch [331]
acetate Titer 5.3 g/L 172 h Fed-Batch [331]
5-hydroxypentanoate Titer 3.72 g/L 172 h Fed-Batch [331]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References