Escherichia coli AC05

Ancestors

Genotype with respect to parental

△gabD△gdhA

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | High lysine-producing strain | Plasmid pLADH added | △gabD△gdhA | Plasmid pLADH lost | Plasmid pBDP added | Plasmid pADCTKC added

Bars (|) indicate differences between strains.

Plasmids

• pBDP (car_{_Mm}-sfp-yqhD-pntAB)

  pADCTKC (p15A ori, P_trc, patAD-cadA-gabT-yahK-cgl0841)

Strain derived from a randomly mutated strain.

References

• Cen, Xuecong; Liu, Yanjuan; Zhu, Fanghuan; Liu, Dehua & Chen, Zhen. Metabolic engineering of Escherichia coli for high production of 1,5-pentanediol via a cadaverine-derived pathway. Metabolic Engineering. 2022, 74, 168-177.