Escherichia coli EPA4 (pDtac15-At2tUH) + pU10099-P_bC

Ancestors

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | P_acuI::P_trc | P_aspC::P_trc | ΔiclR ΔptsG | P_ppc::P_{BBa_J23100} | Plasmid pU10099-P_bC added | Plasmid pDtac15-At2tUH added

Bars (|) indicate differences between strains.

Plasmids

pDtac15-At2tUH (P_tac-act_Cp-acl2_Cp, P_tac-acuI_Ec-ygfH)

pU10099-P_bC (panD_Bs and aspC_Ec)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
propanoate	Titer	1.25 g/L		Glucose		Flask	[337]
propanoate	Titer	14.8 g/L	30.5 OD₆₀₀	Glucose	72 h	Fed-Batch	[337]
propanoate	Substrate yield	0.201 g/g of sustrate	30.5 OD₆₀₀	Glucose	72 h	Fed-Batch	[337]
propanoate	Substrate yield	0.488 mol/mol of substrate	30.5 OD₆₀₀	Glucose	72 h	Fed-Batch	[337]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References