Escherichia coli TrpD-Pl / pCtAAAH-LC / pCOLAJ23-TDC.2

Ancestors

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacU169 gal490 λcI857 Δ(cro-bioA) | rpsL (StrR) | ∆aroF ∆aroG ∆mtr ∆tnaA ∆tnaB ∆λ | ∆aroH::P_{J23119-rpsL-tac} -(aroG^fbr-serA^fbr) | P_trc -trpE^fbrDCBA | ∆TrpR | Plasmid pBbE1k-2.2 added | Plasmid pACPJ23-CtAAAH-LC added | Plasmid pCOLADuet-TDC added

Bars (|) indicate differences between strains.

Plasmids

pBbE1k-2.2 (PCD_Hs and DHPR_Hs)

pACPJ23-CtAAAH-LC (CtAAAH^F197L/E219C)

pCOLADuet-TDC (TDC)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
5-hydroxy-L-tryptophan	Titer	7.3 mg/L	10.2 OD₆₀₀	Glucose	60 h	Fed-Batch	[338]
serotonin	Titer	0.0 mg/L	10.2 OD₆₀₀	Glucose	60 h	Fed-Batch	[338]
L-tryptophan	Titer	5.34 g/L	10.2 OD₆₀₀	Glucose	60 h	Fed-Batch	[338]
tryptamine	Titer	3.03 g/L	10.2 OD₆₀₀	Glucose	60 h	Fed-Batch	[338]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

José-Aníbal Mora-Villalobos & An-Ping Zeng (2018). Synthetic pathways and processes for effective production of 5-hydroxytryptophan and serotonin from glucose in Escherichia coli. Journal of Biological Engineering.