Escherichia coli TrpD-Pl / pCtAAAH-LC / pBAD-TDC
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacU169 gal490 λcI857 Δ(cro-bioA) | rpsL (StrR) | ∆aroF ∆aroG ∆mtr ∆tnaA ∆tnaB ∆λ | ∆aroH::PJ23119-rpsL-tac -(aroGfbr-serAfbr) | Ptrc -trpEfbrDCBA | ∆TrpR | Plasmid pBbE1k-2.2 added | Plasmid pACPJ23-CtAAAH-LC added | Plasmid pBAD-TDC addedBars (|) indicate differences between strains.
Plasmids
-
pBbE1k-2.2 (PCDHs and DHPRHs)
pACPJ23-CtAAAH-LC (CtAAAHF197L/E219C)
pBAD-TDC (pBAD/His, TDC)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| tryptamine | Titer | 1.81 g/L | 60 h | Fed-Batch | [338] | ||
| 5-hydroxy-L-tryptophan | Titer | 21.3 mg/L | 60 h | Fed-Batch | [338] | ||
| serotonin | Titer | 0.8 mg/L | 60 h | Fed-Batch | [338] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.