Escherichia coli PP13
In this strain, the activity of the N-methyltransferase enzyme from Candidatus cloacimonetes was evaluated; however, no psilocin production was detected.
Ancestors
Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | ΔtnaA | Plasmid pMK-CcNMT added | Plasmid pHA-Arg5-KvDDC addedBars (|) indicate differences between strains.
Plasmids
-
pHA-Arg5-KvDDC (PLlacO1-arg5Av-DDCKv-T1)
pMK-CcNMT (NMTCc)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| psilocin | Titer | 0.0 mg/L | 48 h | Flask | [339] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.