Escherichia coli PP26

Ancestors

All strains

Escherichia coli K12

Escherichia coli BW25113

Escherichia coli BW25113ΔtnaA

Escherichia coli PP26

Genotype with respect to wild type

F⁺ (λ) | IacI^q rrnB_T14 ∆lacZ_WJ16 hsdR514 ∆araBAD_AH33 ∆rhaBAD_LD78 | ΔtnaA | Plasmid pMK-LPTG-K added | Plasmid pHA-AD-M added | Plasmid pLC-TrpE^fbrDCBA added

Bars (|) indicate differences between strains.

Plasmids

pHA-AD-M (Arg5-KvDDC, PcPsiM)

pLC-TrpE^fbrDCBA (trpE^fbrDCBA)

pMK-LPTG-K (aroL-ppsA-tktA-aroG^fbr, psiK_Pc)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
psilocybin	Titer	423.56 mg/L		Glycerol	48 h	Flask	[339]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Guo, Cui; Luu, Nguyen N.T.; Adwer, Maryem M.; Hosseinzadeh, Hemen; Balan, Venkatesh; Yan, Yajun & Lin, Yuheng. Engineering Non-Natural Biosynthetic Pathways for Efficient Microbial Production of Psilocybin and Psilocin. Metabolic Engineering. 2025.