Escherichia coli PP27

Ancestors

Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | ΔtnaA | Plasmid pMK-PcPsiK added | Plasmid pHA-AD-M added | Plasmid pLC-TrpEfbrDCBA-LPTG added

Bars (|) indicate differences between strains.


Plasmids

Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
psilocybin Titer 475.88 mg/L 48 h Flask [339]
psilocybin Titer 220.65 mg/L 48 h Flask [339]
psilocybin Titer 557.91 mg/L 48 h Flask [339]
L-tryptophan Titer 191.18 mg/L 48 h Flask [339]
anthranilate Titer 490.11 mg/L 48 h Flask [339]
4-hydroxy-L-tryptophan Titer 0.0 mg/L 48 h Flask [339]
psilocybin Titer 2.0 g/L 75 h Fed-Batch [339]
psilocybin Volumetric productivity 26.7 mg/L/h 75 h Fed-Batch [339]
L-tryptophan Titer 1.07 g/L 75 h Fed-Batch [339]
anthranilate Titer 2.0 g/L 75 h Flask [339]
4-hydroxy-L-tryptophan Titer 0.0 mg/L 75 h Fed-Batch [339]
4-hydroxytryptamine Titer 0.0 mg/L 75 h Fed-Batch [339]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References